SubtiBank SubtiBank
epsN [2018-09-20 18:42:44]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

epsN [2018-09-20 18:42:44]

UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase, required for extracellular polysaccharide synthesis
locus
BSU34230
pI
5.84
mw
42.76 kDa
protein length
388 aa Sequence Blast
gene length
1164 bp Sequence Blast
function
biofilm formation
product
UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase
essential
no
synonyms
yvfE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]

    • Gene

    Coordinates
    3,515,062 → 3,516,228

    Phenotypes of a mutant

  • smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]

    • The protein

    Catalyzed reaction/ biological activity

  • conversion of UDP-2,6-dideoxy 2-acetamido 4-keto glucose to UDP-2,6-dideoxy 2-acetamido 4-amino glucose [pubmed|29982582]

    • Protein family

  • degT/dnrJ/eryC1 family (according to Swiss-Prot)

    • [SW|Cofactors]

  • pyridoxal phosphate [pubmed|29982582]

    • Expression and Regulation

    Operons

    genes
    [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]
    description
    [Pubmed|15661000]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15661000], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: anti-activation, (prevents binding of [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]) [Pubmed|23646920], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [regulon|EAR riboswitch|EAR riboswitch]: processive antitermination, in [regulon|EAR riboswitch|EAR riboswitch]

    • regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|15661000]

    • additional information

  • induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI] or [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] [PubMed|15661000,19788541]
  • expression is increased in [gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX] mutants [pubmed|32483306]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A064 (yvfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/64 NBRP B. subtilis, Japan]
  • BKE34230 (Δ[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA, downstream forward: _UP4_TTCCATACTGTCGAGGTGAA
  • BKK34230 (Δ[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA, downstream forward: _UP4_TTCCATACTGTCGAGGTGAA

    • Labs working on this gene/protein

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

    • References

    Reviews

  • 20735481

    • Research papers

  • 29982582

    • =The EAR [SW|RNA switch]=

  • 20374491,20230605